Advanced Techniques in Molecular Science

To participate in the rapidly expanding fields of genome research and protein structure-function analysis, it is necessary to have an understanding of the techniques used in these areas. This subject provides training in the use of molecular biology technologies of, protein analyses and cell biology. Students will learn how experiments are designed, performed and the resulting data analysed. Experiments in the subject will explore (a) the use of recombinant DNA analyses, (b) bacterial expression systems to produce and characterise recombinant protein, (c) identification of proteins by mass spectrometry; and (d) mammalian cell culture. Students will learn practical skills of how to record data and maintain experimental observations in laboratory notebooks, to search bioinformatic databases, and to construct and concisely write a scientific research paper based on their findings. Students will also further develop their skills in performing biochemical calculations and solving problems by applying knowledge attained from practicals.

深入探讨分子科学领域的高级实验方法。

• 1Describe the importance of key features in the design of experimental protocols used in biochemistry and molecular biology
• 2Demonstrate competence and skill in performing fundamental techniques used in biochemistry and molecular biology
• 3Record data and experimental observations in laboratory notebooks
• 4Design simple experiments and solve problems dealing with scientific information
• 5Use bioinformatics programs to analyse of DNA sequences and protein data derived by mass spectrometry
• 6Use concise written communication to present experiment findings in a scientific report
• 7Critically evaluate scientific literature using database searches.